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Image Search Results
Journal:
Article Title: A CD1d-Dependent Antagonist Inhibits the Activation of Invariant NKT Cells and Prevents Development of Allergen-Induced Airway Hyperreactivity
doi: 10.4049/jimmunol.0901208
Figure Lengend Snippet: DPPE-PEG blocks ERK phosphorylation pathway (FACS histogram for p-ERK1/2 expression in intracellularly stained iNKT lines activated as described in Materials and Methods). The FACS profile shows p-ERK1/2 activation of iNKT cells by APCs pulsed with α-GalCer alone (upper panel) or α-GalCer plus DPPE-PEG (lower panel) at basal rates (time point 0, shaded histograms) and at various time points after incubation (thick lines).
Article Snippet: The mixture was incubated at 37°C for various periods of time, fixed, and stained for intracellular
Techniques: Expressing, Staining, Activation Assay, Incubation